Nūhou

1. Pono e mālama ʻia ka wai hauʻona i loko o kahi pahu wai hauʻoli (liquid nitrogen tank) i hana ʻia e kekahi mea hana mana aupuni, a waiho ʻia i loko o kahi lumi hoʻoluʻu maikaʻi, pouli, a anuanu.

2. Hiki ke ho'opa'a 'ia ka pahu hau hau me ka pahu pahu mua, a he hakahaka ka waha o ka pahu.Ua kapu loa ia e sila i ka waha pahu.A i ʻole, ma muli o ke kaomi nui, hiki ke pahū.

3. E lawe i ka pale pilikino i ka wā e unuhi ai i ka hua hau hau mai ka pahu.He hua haʻahaʻa haʻahaʻa (ka wela -196°).Kāohi i ka hau i ka wā e hoʻohana ai.

4. I mea e hōʻoiaʻiʻo ai i ka motility sperm, pono e hoʻohui ʻia ka nitrogen wai i ka pahu hau hau i ka manawa e hōʻoia ai ʻaʻole hiki ke ʻike ʻia ka sperm paʻa i loko o ka pahu i waho o ka nitrogen wai.

5. E noʻonoʻo i ka pāpaʻi wai a me ka hōʻeha ʻana i nā kānaka.He haʻahaʻa ka helu hoʻolapalapa o ka wai nitrogen.Ke hālāwai me nā mea i ʻoi aku ka kiʻekiʻe ma mua o kona wela (maʻamau maʻamau), e hoʻolapalapa, e mahu, a i ʻole e paʻi.

6. E nānā pinepine i ka hana ʻana o ka pahu hau hau.Inā ʻike ʻia ka pahu hau hau ma ka ʻili o ka pahu pahu a i ʻole ka pahu wai nitrogen me ka maikaʻi ʻole o ka hana ʻana i ka hoʻoheheʻe wela i ka wā e hoʻohana ai, pono e hoʻōki a hoʻololi koke ʻia.

7. Ma muli o kāna hana pono ʻana a me nā hiʻohiʻona kūlohelohe, ʻaʻole ʻae ʻia nā pahu wai nitrogen wai e hoʻokuʻi ʻia, waiho ʻia i ka pae ākea, hoʻohuli ʻia, hoʻopaʻa ʻia, hui pū kekahi i kekahi a i ʻole e hui pū me nā mea ʻē aʻe i ka wā o ka lawe ʻana a me ka mālama ʻana.E ʻoluʻolu e lawelawe me ka mālama a e kū pololei.ʻO ka mea nui, pono e hoʻopaʻa ʻia i ka wā o ka lawe ʻana i mea e pale ai i ka poʻe i ka hau hau a i ʻole nā ​​​​mea hana ma hope o ka hoʻolei ʻana i ka wai hau.

8. No ka mea, ʻaʻole he bactericidal ka waiʻona wai, pono e nānā ʻia ka disinfection o nā mea hana e pili ana me ka wai nitrogen.